Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp- 1 of SARS-CoV spike protein
Identifieur interne : 005B29 ( Main/Exploration ); précédent : 005B28; suivant : 005B30Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp- 1 of SARS-CoV spike protein
Auteurs : BAOMEI WANG [République populaire de Chine] ; HUABIAO CHEN ; XIAODONG JIANG ; MINGHUI ZHANG ; TAO WAN ; NAN LI ; XIANGYANG ZHOU ; YANFENG WU ; FENG YANG ; YIZHI YU ; XIAONING WANG ; RUIFU YANG ; XUETAO CAOSource :
- Blood [ 0006-4971 ] ; 2004.
Descripteurs français
- Pascal (Inist)
- Syndrome respiratoire aigu sévère, Coronavirus, Glycoprotéine, Déterminant antigénique, Peptide, Système histocompatibilité majeur, Système HLA, Antigène histocompatibilité classe I, Locus HLA-A, Restriction histocompatibilité, Lymphocyte T, Lymphocyte T cytotoxique, Réponse immune, Animal transgénique, Souris, Antigène CD8, Protéine SSp-1.
- Wicri :
- topic : Animal transgénique.
English descriptors
- KwdEn :
- Antigenic determinant, Class I histocompatibility antigen, Coronavirus, Cytotoxic T lymphocyte, Glycoprotein, HLA-A-Locus, HLA-System, Histocompatibility restriction, Immune response, Major histocompatibility system, Mouse, Peptides, Severe acute respiratory syndrome, T-Lymphocyte, Transgenic animal.
Abstract
A novel coronavirus, severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV), has been identified as the causal agent of SARS. Spike (S) protein is a major structural glycoprotein of the SARS virus and a potential target for SARS-specific cell-mediated immune responses. A panel of S protein-derived peptides was tested for their binding affinity to HLA-A*0201 molecules. Peptides with high affinity for HLA-A*0201 were then assessed for their capacity to elicit specific immune responses mediated by cytotoxic T lymphocytes (CTLs) both in vivo, in HLA-A2.1/Kb transgenic mice, and in vitro, from peripheral blood lymphocytes (PBLs) sourced from healthy HLA-A2.1+ donors. SARS-CoV protein-derived peptide-1 (SSp-1 RLNEVAKNL), induced peptide-specific CTLs both in vivo (transgenic mice) and in vitro (human PBLs), which specifically released interferon-γ (IFN-γ) upon stimulation with SSp-1-pulsed autologous dendritic cells (DCs) or T2 cells. SSp-1-specific CTLs also lysed major histocompatibility complex (MHC)-matched tumor cell lines engineered to express S proteins. HLA-A*0201-SSp-1 tetramer staining revealed the presence of significant populations of SSp-1-specific CTLs in SSp-1-induced CD8+ T cells. We propose that the newly identified epitope SSp-1 will help in the characterization of virus control mechanisms and immunopathology in SARS-CoV infection, and may be relevant to the development of immunotherapeutic approaches for SARS.
Affiliations:
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protein</title><author><name sortKey="Baomei Wang" sort="Baomei Wang" uniqKey="Baomei Wang" last="Baomei Wang">BAOMEI WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute of Immunology, Second Military Medical University</s1><s2>Shanghai</s2><s3>CHN</s3></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Shanghai</wicri:noRegion></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Institute of Immunology, Zhejiang University</s1><s2>Hangzhou</s2><s3>CHN</s3></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Institute of Molecular Immunology, First Military Medical University</s1><s2>Guangzhou</s2><s3>CHN</s3></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences</s1><s2>Beijing</s2><s3>CHN</s3></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Huabiao Chen" sort="Huabiao Chen" uniqKey="Huabiao Chen" last="Huabiao Chen">HUABIAO CHEN</name></author><author><name sortKey="Xiaodong Jiang" sort="Xiaodong Jiang" uniqKey="Xiaodong Jiang" last="Xiaodong Jiang">XIAODONG JIANG</name></author><author><name sortKey="Minghui Zhang" sort="Minghui Zhang" uniqKey="Minghui Zhang" last="Minghui Zhang">MINGHUI ZHANG</name></author><author><name sortKey="Tao Wan" sort="Tao Wan" uniqKey="Tao Wan" last="Tao Wan">TAO WAN</name></author><author><name sortKey="Nan Li" sort="Nan Li" uniqKey="Nan Li" last="Nan Li">NAN LI</name></author><author><name sortKey="Xiangyang Zhou" sort="Xiangyang Zhou" uniqKey="Xiangyang Zhou" last="Xiangyang Zhou">XIANGYANG ZHOU</name></author><author><name sortKey="Yanfeng Wu" sort="Yanfeng Wu" uniqKey="Yanfeng Wu" last="Yanfeng Wu">YANFENG WU</name></author><author><name sortKey="Feng Yang" sort="Feng Yang" uniqKey="Feng Yang" last="Feng Yang">FENG YANG</name></author><author><name sortKey="Yizhi Yu" sort="Yizhi Yu" uniqKey="Yizhi Yu" last="Yizhi Yu">YIZHI YU</name></author><author><name sortKey="Xiaoning Wang" sort="Xiaoning Wang" uniqKey="Xiaoning Wang" last="Xiaoning Wang">XIAONING WANG</name></author><author><name sortKey="Ruifu Yang" sort="Ruifu Yang" uniqKey="Ruifu Yang" last="Ruifu Yang">RUIFU YANG</name></author><author><name sortKey="Xuetao Cao" sort="Xuetao Cao" uniqKey="Xuetao Cao" last="Xuetao Cao">XUETAO CAO</name></author></analytic><series><title level="j" type="main">Blood</title><title level="j" type="abbreviated">Blood</title><idno type="ISSN">0006-4971</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Blood</title><title level="j" type="abbreviated">Blood</title><idno type="ISSN">0006-4971</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigenic determinant</term><term>Class I histocompatibility antigen</term><term>Coronavirus</term><term>Cytotoxic T lymphocyte</term><term>Glycoprotein</term><term>HLA-A-Locus</term><term>HLA-System</term><term>Histocompatibility restriction</term><term>Immune response</term><term>Major histocompatibility system</term><term>Mouse</term><term>Peptides</term><term>Severe acute respiratory syndrome</term><term>T-Lymphocyte</term><term>Transgenic animal</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Coronavirus</term><term>Glycoprotéine</term><term>Déterminant antigénique</term><term>Peptide</term><term>Système histocompatibilité majeur</term><term>Système HLA</term><term>Antigène histocompatibilité classe I</term><term>Locus HLA-A</term><term>Restriction histocompatibilité</term><term>Lymphocyte T</term><term>Lymphocyte T cytotoxique</term><term>Réponse immune</term><term>Animal transgénique</term><term>Souris</term><term>Antigène CD8</term><term>Protéine SSp-1</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Animal transgénique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel coronavirus, severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV), has been identified as the causal agent of SARS. Spike (S) protein is a major structural glycoprotein of the SARS virus and a potential target for SARS-specific cell-mediated immune responses. A panel of S protein-derived peptides was tested for their binding affinity to HLA-A*0201 molecules. Peptides with high affinity for HLA-A*0201 were then assessed for their capacity to elicit specific immune responses mediated by cytotoxic T lymphocytes (CTLs) both in vivo, in HLA-A2.1/K<sup>b</sup> transgenic mice, and in vitro, from peripheral blood lymphocytes (PBLs) sourced from healthy HLA-A2.1<sup>+</sup> donors. SARS-CoV protein-derived peptide-1 (SSp-1 RLNEVAKNL), induced peptide-specific CTLs both in vivo (transgenic mice) and in vitro (human PBLs), which specifically released interferon-γ (IFN-γ) upon stimulation with SSp-1-pulsed autologous dendritic cells (DCs) or T2 cells. SSp-1-specific CTLs also lysed major histocompatibility complex (MHC)-matched tumor cell lines engineered to express S proteins. HLA-A*0201-SSp-1 tetramer staining revealed the presence of significant populations of SSp-1-specific CTLs in SSp-1-induced CD8<sup>+</sup> T cells. We propose that the newly identified epitope SSp-1 will help in the characterization of virus control mechanisms and immunopathology in SARS-CoV infection, and may be relevant to the development of immunotherapeutic approaches for SARS.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li><li>Zhejiang</li></region><settlement><li>Hangzhou</li><li>Jiangmen</li><li>Pékin</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><noCountry><name sortKey="Feng Yang" sort="Feng Yang" uniqKey="Feng Yang" last="Feng Yang">FENG YANG</name><name sortKey="Huabiao Chen" sort="Huabiao Chen" uniqKey="Huabiao Chen" last="Huabiao Chen">HUABIAO CHEN</name><name sortKey="Minghui Zhang" sort="Minghui Zhang" uniqKey="Minghui Zhang" last="Minghui Zhang">MINGHUI ZHANG</name><name sortKey="Nan Li" sort="Nan Li" uniqKey="Nan Li" last="Nan Li">NAN LI</name><name sortKey="Ruifu Yang" sort="Ruifu Yang" uniqKey="Ruifu Yang" last="Ruifu Yang">RUIFU YANG</name><name sortKey="Tao Wan" sort="Tao Wan" uniqKey="Tao Wan" last="Tao Wan">TAO WAN</name><name sortKey="Xiangyang Zhou" sort="Xiangyang Zhou" uniqKey="Xiangyang Zhou" last="Xiangyang Zhou">XIANGYANG ZHOU</name><name sortKey="Xiaodong Jiang" sort="Xiaodong Jiang" uniqKey="Xiaodong Jiang" last="Xiaodong Jiang">XIAODONG JIANG</name><name sortKey="Xiaoning Wang" sort="Xiaoning Wang" uniqKey="Xiaoning Wang" last="Xiaoning Wang">XIAONING WANG</name><name sortKey="Xuetao Cao" sort="Xuetao Cao" uniqKey="Xuetao Cao" last="Xuetao Cao">XUETAO CAO</name><name sortKey="Yanfeng Wu" sort="Yanfeng Wu" uniqKey="Yanfeng Wu" last="Yanfeng Wu">YANFENG WU</name><name sortKey="Yizhi Yu" sort="Yizhi Yu" uniqKey="Yizhi Yu" last="Yizhi Yu">YIZHI YU</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Baomei Wang" sort="Baomei Wang" uniqKey="Baomei Wang" last="Baomei Wang">BAOMEI WANG</name></noRegion><name sortKey="Baomei Wang" sort="Baomei Wang" uniqKey="Baomei Wang" last="Baomei Wang">BAOMEI WANG</name><name sortKey="Baomei Wang" sort="Baomei Wang" uniqKey="Baomei Wang" last="Baomei Wang">BAOMEI WANG</name><name sortKey="Baomei Wang" sort="Baomei Wang" uniqKey="Baomei Wang" last="Baomei Wang">BAOMEI WANG</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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